U87-MG/Luciferase cells are derived from the human glioblastoma cell line U87-MG by stably integration of a constitutive Firefly luciferase expression construct. The U87-MG cell line has been widely used in cancer research and drug development. U87-MG/Luciferase cells express Firefly luciferase under the control of the CMV promoter, can be used for in vitro assays and in vivo imaging.