Lentivirus Production

Catalog number
CLV
Description

Get ready-to-use custom lentivirus in two weeks. Use your own construct or let FenicsBIO generate the construct for you. Select from our standard production scales or request a quote for custom scale. All custom lentivirus will be:

  • Functionally titrated, titer guaranteed
  • Serum-free, purity high enough for both in vitro and in vivo use
  • Stored in LentiShieldTM lentivirus storage buffer to prevent titer loss during freeze-thaw cycles

To order

  1. CLV-106

    Lentivirus Production-Small scale

    10^6 TU/ml, 10 ml
    $390.00
  2. CLV-108

    Lentivirus Production-Standard scale

    >1x10^8 TU/ml, 100 ul (4x25ul)
    $980.00
  3. CLV-109

    Lentivirus Production-Large scale

    >1x10^9 TU/ml, 200ul (8x25ul)
    $1,990.00

Overview

FenicsBIO's Custom Lentivirus Production Service uses all of our innovative products for every step of lentivirus production process to ensure you get ready-to-use lentivirus with highest quality.

Why choose FenicsBIO?

Superior services:

  • Fast turnaround, custom lentivirus ready in two weeks. 
  • Flexible production scales. 
  • Expert technical support covering every step of your project.
  • Dedicated project manager, weekly progress report.
  • Strict quality control.

 Highest quality lentivirus:

  • Serum-free, purity high enough for both in vitro and in vivo use. 
  • Functional titer from mammalian cells
  • Stored in LentishieldTM lentivirus storage buffer to prevent titer loss during freeze-thaw cycles. 

Our default lentivirus production services produce VSV-G pseudotyped lentiviruses that integrate into the host genome. If you need lentivirus with other envelope including SARS-CoV-2 spike proteinMuLV, and Rabies or need non-integrating lentiviruses, please specify in your project requirements. 

Deliverables/Lead Time

 

SKU Lentivirus Titer Deliverables Lead Time
CLV-106 ~10^6 TU/ml 10 ml lentivirus supernatant 1 week
CLV-108 >1x10^8 TU/ml 100ul (4x25ul) purified and concentrated lentivirus 2 weeks
CLV-109 >1x10^9 TU/ml 200ul (8x25ul) purified and concentrated lentivirus 2 weeks
CLV-custom Custom Custom Request a quote

                        TU=Transduction units. All titers are determined by lentivirus titration in mammalian cells, typically in HT1080                                      and HEK293T cells. 

 

QC Testing

Our lentivirus quality control includes:

  • Sterile test
  • Functional titration on mammalian cells. Depending on the lentiviral constructs, functional titration readout can be counting antibiotic resistant cell clones, counting fluorescent positive cell clones or genomic real-time PCR.
  • If you need additional quality control tests for your lentivirus production project, please specify in your project requirements.

Project Initiation

Starting lentivirus production service is easy and flexible.

If you use your own construct, please send us at least 1ug of the plasmid DNA, and plasmid map/sequence.

If you need FenicsBIO to generate the construct, your project manager will communicate with you to discuss your requirements of the construct, such as promoter to use, antibiotic selection gene, marker et al. You can select the vector from our Lentiviral cloning vectors or send us your own vector. 

 

Supporting Documents

Lentivirus Production

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