TF-1/iRFP cells are derived from the human erythroleukemia cell line TF-1 by stably integration of a constitutive miRFP670 stably expression construct. TF-1 cells respond to a variety of cytokines, including interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), are ideal for studying how cytokines regulate hematopoietic cell growth, proliferation, and differentiation. TF-1 cells have been widely used to screen for anti-leukemic drugs or compounds that target cytokine receptors, growth factor signaling pathways, or other molecular mechanisms involved in hematopoietic cancer. TF-1/iRFP cells stably stably express miRFP670, can be used for in vitro assays and in vivo imaging.