RS4;11/Luciferase cells are derived from the human RS4;11 lymphoblast cell line by stably integration of a constitutive Firefly luciferase expression construct. RS4;11 cell line was generated from acute lymphoblastic leukemia, has been widely used in cancer research and drug development. RS4;11/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.