RPMI-8226/Luciferase cells are derived from the human myeloma RPMI-8226 cell line by stably integration of a constitutive Firefly luciferase expression construct. RPMI-8226 cells have been used in cancer research and drug development. RPMI-8226/Luciferase cells express Firefly luciferase under the control of the CMV promoter, can be used for in vitro assays and in vivo imaging.