RPMI-8226/GFP-luciferase cells are derived from the human myeloma RPMI-8226 cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. The RPMI-8226 cell line is a valuable tool for understanding multiple myeloma and developing new treatments, including CAR-T (chimeric antigen receptor T-cell) therapy. RPMI-8226/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.