RPMI-8226/GFP-luciferase cells are derived from the human myeloma RPMI-8226 cell line by stably integration of a constitutive GFP and Firefly luciferase expression construct. RPMI-8226 cells have been used in cancer research and drug development. RPMI-8226/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.