Overview

Inducible gene expression systems, such as Tet-On and Tet-Off systems, make “on demand” gene expression possible. The gene expression can be controlled spatiotemporally.

The Tet-Off and Tet-On expression systems are binary transgenic systems in which expression from a target transgene is dependent on the activity of an inducible transcriptional activator (tTA for Tet-Off system and rtTA for Tet-On system) binding to Tet Responsive Element (TRE) at the promoter.

The activity of tTA and rtTA is regulated by the presence or absence of tetracycline or one of its derivatives such as doxycycline. In Tet-Off system, binding to tetracycline prevents the tTA from binding to TRE, results in gene expression inhibition. In Tet-On system, binding to tetracycline allows rtTA to bind to TRE, results in induced gene expression. In both the Tet-Off and Tet-On systems, expression of the transcriptional activator can be regulated both reversibly and quantitatively by varying concentrations of tetracycline or doxycycline (Dox).

To facilitate your research work, FenicsBIO provides lentiviruses with Tet inducible cDNA, shRNA,  miRNA, tTA and rtTA driven by different promoters.

Resources

* These products are covered by a series of patents. Researchers at not-for-profit organizations receive a limited use license with  purchase of the products. For profit entities are required to obtain a license. Please check license statement for details.