Overview
CRIPSR/Cas9 has been widely used for gene knockout in mammalian cells. Using unique algorithms, we developed a set of three spatially coordinated sgRNAs and a pair of Indel PCR primers for each human gene. The three sgRNAs cause a large gragment deletion, making it easier to be detected by genomic PCR, greatly improved gene knockout efficacy. Provided Indel PCR primers are optimized for gene specificity, making it easy to screen gene knockout cell clones before sequencing. Our sgRNA knockout kit guarantees gene knockout. With large selection of premade Cas9 lentiviruses with different promoters and selection markers, we provide flexibility for optimizing Cas9 gene expression in almost all types of mammalian cells to make gene knoutkout more efficient. By combination with tissue specific expressed Cas9, this kit can be used for gene knockout in specific cell types in vivo.
- Premade lentiviral sgRNA particles ready for gene knockout
- Highly efficient gene knockout with a set of three sgRNAs- Three sgRNAs lead to a deletion in cDNA coding region.
- Tissue-specific gene knockout in vivo- Achieve tissue-specific gene knockout when using together with tissue-specific expressed Cas9.
- Screening Indel PCR primers included- Compared with wild type, gene knockout cell lines show a truncated PCR product.
Product Selection
FenicsBIO’s lentiviral sgRNAs and Cas9 are provided on two separated vectors for optimized Cas9 expression and the flexibility of knocking out genes in specific tissues in vivo. Use the table below to help you decide which Cas9 to use together with your sgRNAs.
| Cas9 with constitutive promoters | Cas9 with tet inducible promoter | Cas9 with tissue specific promoter | |
| For easy to transfect cells | Cas9 plasmids | Cas9 plasmids | Cas9 plasmids |
| For hard to transfect cells | Cas9 Lentiviral particles | Cas9 Lentiviral particles | Cas9 Lentiviral particles |
| Vector format | Lentiviral | Lentiviral | Lentiviral |
| Promoter | Choice of four constitutive promoters | Tet inducible promoter | Tissue-specific promoters |
| Inducible expression | No | Yes | No |
| Tissue specific knockout | No | Yes, when used with tissue specific expressed rtTA | Yes |
| Applicable cells | Almost all types of mammalian cells | Almost all types of mammalian cells | Almost all types of mammalian cells |
| Create knockout cell line | Yes | Yes | Yes |
| In vivo use |
Yes |
Yes |
Yes |
Guarantee
With a set of three sgRNAs, FenicsBIO guarantee gene knockout. Optimal transfection/trasduction conditions and Cas9 expression level has to be optimized for specific cell types.