Raw264.7/GFP-luciferase cells are derived from the mouse macrophage cell line Raw264.7 by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. Raw264.7 cells have been used as a model for studying the response of inflammatory molecules to various stimuli and evaluating the effects of anti-inflammatory drugs. Raw264.7/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.