Overview
The Synapsin promoter comes from the human Synapsin I gene. This gene is key in controlling neurotransmitter release and is mainly active in neurons. Lentiviral shRNA driven by the Synapsin promoter is useful for specifically expressing shRNA in neurons. This is especially helpful for studying gene function in neuronal cells, whether in vitro or in vivo. It enables precise gene knockdown in neurons, preventing silencing in other cell types.
- Neuron specific expression-Allowing neuron specific gene knockdown in vivo.
- Third generation lentiviral vectors-Safest, no replication competent lentivirus (RCL) found.
- Plasmids or purified lentiviral particles for a set of three gene-specific shRNA and one control shRNA -Plasmids are transfection-ready. Lentiviral particles are concentrated and purified, with functional titer of 1x10^8 TU/ml.
- Pre-designed Real-time RT-PCR primers included for verification-Unique design algorithm for specific RT-PCR products.
- Guarantee gene knockdown-At least one of the three shRNAs knockdown gene expression by at least 70%.
Guarantee
With a set of three shRNAs, FenicsBIO guarantees at least one will knockdown gene expression by 70% when validating with the real-time PCR primers provided.