Overview
The efficacy of shRNA gene knockdown is not only depending on the shRNA sequence design, but also affected by the expression level of shRNA. Therefore, selecting the right promoter to drive shRNA expression is critical for achieving successful gene knockdown. We suggest using our Promoter selection plate to test which promoter works best for your cells before moving forward with shRNA knockdown experiments.
- Choice of four constitutive promoters-Tissue specific promoter available upon request.
- Third generation lentiviral vectors-Safest, no replication competent lentivirus (RCL) found.
- Plasmids or purified lentiviral particles for a set of three gene-specific shRNA and one control shRNA -Plasmids are transfection-ready. Lentiviral particles are concentrated and purified, with functional titer of 1x10^8 TU/ml.
- Pre-designed Real-time RT-PCR primers included for verification-Unique design algorithm for specific RT-PCR products.
- Guarantee gene knockdown-At least one of the three shRNAs knockdown gene expression by at least 70%.
Controls
Control shRNA with CMV promoter
Control shRNA with EF1a promoter
Guarantee
With a set of three shRNAs, FenicsBIO guarantees at least one will knockdown gene expression by 70% when validating with the real-time PCR primers provided. Optimal promoter has to be optimized for specific cell types.