PC-3/iRFP cells are derived from the human prostate cancer PC-3 cell line by stably integration of a constitutive miRFP670 stably expression construct. PC3 cells are known for being androgen-independent, making them a relevant model for studying advanced prostate cancer. PC-3/iRFP cells stably express miRFP670, can be used for in vitro assays and in vivo imaging.