Panc 02.03/RFP-Luciferase cells are derived from the human pancreatic cancer cell line Panc 02.03 by stably integration of a constitutive RFP-Firefly luciferase stably expression construct. Panc 02.03 cells have been used in cancer research and drug development. Panc 02.03/RFP-Luciferse cells stably stably express RFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.