OVCAR-3/RFP-Luciferase cells are derived from the human ovarian adenocarcinoma cell line OVCAR-3 by stably integration of a constitutive RFP-Firefly luciferase stably expression construct. OVCAR-3 cells are known to express high levels of the estrogen receptor (ER) and progesterone receptor (PR), are sensitive to hormones such as estradiol and progesterone, and have been used to investigate hormone-related signaling pathways and treatments. OVCAR-3/RFP-Luciferse cells stably stably express RFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.