NIH3T3/RFP-Luciferase cells are derived from the mouse embryonic fibroblast cell line NIH3T3 by stably integration of a constitutive RFP-Firefly luciferase stably expression construct. NIH3T3 cells have been have been widely used in biomedical research. NIH3T3/RFP-Luciferse cells stably stably express RFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.