NIH3T3/Luciferase cells are derived from the mouse embryonic fibroblast cell line NIH3T3 by stably integration of a constitutive Firefly luciferase stably expression construct. NIH3T3 cells have been have been widely used in biomedical research. NIH3T3/Luciferase cells stably express firefly luciferase, can be used for in vitro assays and in vivo imaging.