NIH3T3/Cre-GFP cells are derived from the mouse embryonic fibroblast cell line NIH3T3 by stably integration of a constitutive GFP fused Cre recombinase stably expression construct. NIH3T3 cells have been have been widely used in biomedical research. NIH3T3/Cre-GFP cells stably express a GFP fused nuclear localized Cre recombinase.