The NFAT RFP reporter lentivirus (Hyg) is purified, funtional titrtated VSV-G peudotyped lentivirus ready-to-use for transducing almost all types of mammalian cells. This lentivirus expresses RFP under the control of tandem NFAT responsive elements and a minimal promoter, can be used to monitor NFAT signal by GFP fluorescence. With a hygromycin selection marker, the NFAT RFP reporter lentivirus can be used to generate NFAT reporter cell lines.