The NFAT RFP-luciferase reporter lentivirus (Puro) is purified, funtional titrtated VSV-G peudotyped lentivirus ready-to-use for transducing almost all types of mammalian cells. This lentivirus expresses RFP and firefly luciferase under the control of tandem NFAT responsive elements and a minimal promoter, can be used to monitor NFAT signal by both GFP fluorescence and firefly luciferase signals. With a puromycin selection marker, the NFAT GFP-luciferase reporter lentivirus can be used to generate NFAT reporter cell lines.