NCI-H460/Luciferase cells are derived from the human NCI-H460 lung carcinoma cell line by stably integration of a constitutive Firefly luciferase stably expression construct. NCI-H460 cell line has been widely used in cancer research and drug development. NCI-H460/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.