NCI-H441/iRFP cells are derived from the human NCI-H441 lung carcinoma cell line by stably integration of a constitutive miRFP670 stably expression construct. NCI-H441 cell line is a useful model for understanding the complexities of lung cancer and for the development of new therapeutic strategies. NCI-H441/iRFP cells stably stably express miRFP670, can be used for in vitro assays and in vivo imaging.