NCI-H358 SMAD/TGFbeta Reporter (Luc) cells are derived from the human non-small cell lung cancer (NSCLC) NCI-H358 cell line by stably integration of a SMAD/TGFbeta firefly luciferase reporter construct. The NCI-H358 cell line is notable for its mutations in the KRAS gene. NCI-H358 cells have been used to study mechanisms of cancer biology, particularly those related to KRAS signaling, as well as to evaluate the efficacy of targeted therapies and novel compounds against KRAS-mutant cancers. NCI-H358 SMAD/TGFbeta Reporter (Luc) cells stably express firefly luciferase under the control of the SMAD/TGFbeta response elements, can be used for monitoring the activity of TGFβ/SMAD signaling pathway.