NALM6/RFP cells are derived from the human precursor B cell lymphoblast cell line NALM6 by stably integration of a constitutive RFP stably expression construct. NALM6 cells stably express CD19, are ideal for testing CAR T cell or other immunotherapies. NALM6/RFP cells stably stably express RFP, can be used for in vitro assays and in vivo imaging.