NALM-1/Luciferase cells are derived from the human NALM-1 macrophage cell line by stably integration of a constitutive Firefly luciferase stably expression construct. NALM-1 cell line was generated from biphenotypic B myelomonocytic leukemia, has been widely used in cancer research and drug development. NALM-1/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.