MV4-11/Luciferase cells are derived from the human MV4-11 macrophage cell line by stably integration of a constitutive Firefly luciferase stably expression construct. MV4-11 cell line was generated from biphenotypic B myelomonocytic leukemia, has been widely used in cancer research and drug development. MV4-11/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.