MV4-11/GFP-luciferase cells are derived from the human MV4-11 macrophage cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. MV4-11 cell line was generated from biphenotypic B myelomonocytic leukemia, has been widely used in cancer research and drug development. MV4-11/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.