MRC5/GFP-luciferase cells are derived from he human fibroblast cell line MRC5 by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. MRC5 cells are widely used in the studies of cell biology, virus biology, cellular aging and toxicology. MRC5/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.