The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are frequently silenced in advanced cancer and have been implicated in epithelial to mesenchymal transition (EMT) and tumor invasion by targeting the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. miR-200a lentivirus (TET-inducible, Puro) expresses mouse miR-200a under the control of the Tet responsive promoter, with puromycin as a selection marker.
