MIAPaCa-2/RFP-Luciferase cells are derived from the human pancreas carcinoma MIA PaCa-2 cell line by stably integration of a constitutive RFP-Firefly luciferase stably expression construct. MIA PaCa-2 cells are widely used to study pancreatic cancer biology and to evaluate the effectiveness of new drugs and targeted therapies for treating pancreatic cancer. MIAPaCa-2/RFP-Luciferase cells stably stably express RFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.