MCA-205/GFP-luciferase cells are derived from the mouse fibrosarcoma cell line MCA-205 by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. MCA-205 cells are widely used for studying tumor-reactive T lymphocytes, the most critical requirement for adoptive immunotherapy. MCA-205/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.