LN229/RFP-Luciferase cells are derived from the human glioblastoma cell line LN229 by stably integration of a constitutive RFP-Firefly luciferase stably expression construct. The LN229 cell line has been widely used in studies to understand the biology of glioblastoma and to test potential treatments. LN229/RFP-Luciferase cells stably stably express RFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.