To order
Overview
FenicsBIO's Custom Lentivirus Production Service uses all of our innovative products for every step of lentivirus production process to ensure you get ready-to-use lentivirus with highest quality.
Why choose FenicsBIO?
Superior services:
- Fast turnaround, custom lentivirus ready in two weeks.
- Flexible production scales.
- Expert technical support covering every step of your project.
- Dedicated project manager, weekly progress report.
- Strict quality control.
Highest quality lentivirus:
- Serum-free, purity high enough for both in vitro and in vivo use.
- Functional titer from mammalian cells
- Stored in LentishieldTM lentivirus storage buffer to prevent titer loss during freeze-thaw cycles.
Our default lentivirus production services produce VSV-G pseudotyped lentiviruses that integrate into the host genome. If you need lentivirus with other envelope including SARS-CoV-2 spike protein, MuLV, and Rabies or need non-integrating lentiviruses, please specify in your project requirements.
Deliverables/Lead Time
SKU | Lentivirus Titer | Deliverables | Lead Time |
CLV-106 | ~10^6 TU/ml | 10 ml lentivirus supernatant | 1 week |
CLV-108 | >1x10^8 TU/ml | 100ul (4x25ul) purified and concentrated lentivirus | 2 weeks |
CLV-109 | >1x10^9 TU/ml | 200ul (8x25ul) purified and concentrated lentivirus | 2 weeks |
CLV-custom | Custom | Custom | Request a quote |
TU=Transduction units. All titers are determined by lentivirus titration in mammalian cells, typically in HT1080 and HEK293T cells.
QC Testing
Our lentivirus quality control includes:
- Sterile test
- Functional titration on mammalian cells. Depending on the lentiviral constructs, functional titration readout can be counting antibiotic resistant cell clones, counting fluorescent positive cell clones or genomic real-time PCR.
- If you need additional quality control tests for your lentivirus production project, please specify in your project requirements.
Project Initiation
Starting lentivirus production service is easy and flexible.
If you use your own construct, please send us at least 1ug of the plasmid DNA, and plasmid map/sequence.
If you need FenicsBIO to generate the construct, your project manager will communicate with you to discuss your requirements of the construct, such as promoter to use, antibiotic selection gene, marker et al. You can select the vector from our Lentiviral cloning vectors or send us your own vector.