Lentiviral human SPARC sgRNA gene knockout kit

Catalog number

HKO-618782

Kit Components

Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers

To order

HKO-618782-25
Lentiviral human SPARC sgRNA gene knockout kit (25)
Lentiviral particles for a set of three sgRNAs mixture (10^8 TU/ml, 25 ul), and a pair of Indel screening PCR primers (100 reactions)
$499.00
HKO-618782-100
Lentiviral human SPARC sgRNA gene knockout kit (100)
Lentiviral particles for a set of three sgRNAs mixture (10^8 TU/ml, 100 ul), and a pair of Indel screening PCR primers (200 reactions)
$890.00
HKO-618782-10ug
Lentiviral human SPARC sgRNA gene knockout kit (plasmid)
Lentiviral plasmids for a set of three sgRNAs (10ug each), and a pair of Indel screening PCR primers (200 reactions)
$890.00

Gene symbol	SPARC
Synonyms	BM-40 OI17 ON ONT
Species	HUMAN
Gene ID	6678
Summary	This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015].
NM numbers targeted (Accession number:Exon:CDS Fragment)	NM_003118:5:4 NM_001309443:5:4 NM_001309444:5:4

Functional titer	1x10^8 TU/ml
Titration method	counting puromycin resistant cell clones
Purity	In vivo grade
Pseudotype	VSV-G
Storage buffer	LentiShield™Lentivirus storage buffer
Storage condition	-80°C
Availability	3-5 weeks

Target sgRNA	Human SPARC gRNA
Promoter for target sgRNA	U6
Mammalian selection	puromycin
Promoter for mammalian selection gene	EF1a
E.coli selection	Amp
Storage condition	-20°C
Availability	1-2 weeks