Lentiviral human RAPH1 sgRNA gene knockout kit

Catalog number

HKO-617556

Kit Components

Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers

To order

HKO-617556-25
Lentiviral human RAPH1 sgRNA gene knockout kit (25)
Lentiviral particles for a set of three sgRNAs mixture (10^8 TU/ml, 25 ul), and a pair of Indel screening PCR primers (100 reactions)
$499.00
HKO-617556-100
Lentiviral human RAPH1 sgRNA gene knockout kit (100)
Lentiviral particles for a set of three sgRNAs mixture (10^8 TU/ml, 100 ul), and a pair of Indel screening PCR primers (200 reactions)
$890.00
HKO-617556-10ug
Lentiviral human RAPH1 sgRNA gene knockout kit (plasmid)
Lentiviral plasmids for a set of three sgRNAs (10ug each), and a pair of Indel screening PCR primers (200 reactions)
$890.00

Gene symbol	RAPH1
Synonyms	ALS2CR18 ALS2CR9 LPD PREL-2 PREL2 RalGDS/AF-6 RMO1
Species	HUMAN
Gene ID	65059
Summary	This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016].
NM numbers targeted (Accession number:Exon:CDS Fragment)	NM_213589:4:3 NM_203365:4:3 NM_001329728:4:3

Functional titer	1x10^8 TU/ml
Titration method	counting puromycin resistant cell clones
Purity	In vivo grade
Pseudotype	VSV-G
Storage buffer	LentiShield™Lentivirus storage buffer
Storage condition	-80°C
Availability	3-5 weeks

Target sgRNA	Human RAPH1 gRNA
Promoter for target sgRNA	U6
Mammalian selection	puromycin
Promoter for mammalian selection gene	EF1a
E.coli selection	Amp
Storage condition	-20°C
Availability	1-2 weeks