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Lentiviral human MCFD2 shRNA (UAS)

Catalog number
HSH-115670
Kit Components
Lentiviral particles or plasmids for 3 unique target specific shRNA and 1 scramble shRNA, RT-PCR primer mix for target specific gene and a housekeeping gene
Lentiviral human MCFD2 shRNA (UAS)
To order Product Details

To order

  1. HSH-115670-25

    Lentiviral human MCFD2 shRNA (UAS) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  2. HSH-115670-100

    Lentiviral human MCFD2 shRNA (UAS) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  3. HSH-115670-10ug

    Lentiviral human MCFD2 shRNA (UAS) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 10 ug each
    $1,290.00
  4. HSH-115670-25GFP

    Lentiviral human MCFD2 shRNA (UAS,GFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  5. HSH-115670-100GFP

    Lentiviral human MCFD2 shRNA (UAS,GFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  6. HSH-115670-10ugGFP

    Lentiviral human MCFD2 shRNA (UAS,GFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 10 ug each
    $1,290.00
  7. HSH-115670-25RFP

    Lentiviral human MCFD2 shRNA (UAS,RFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  8. HSH-115670-100RFP

    Lentiviral human MCFD2 shRNA (UAS,RFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  9. HSH-115670-10ugRFP

    Lentiviral human MCFD2 shRNA (UAS,RFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 10 ug each
    $1,290.00
  10. HSH-115670-25iRFP

    Lentiviral human MCFD2 shRNA (UAS,iRFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  11. HSH-115670-100iRFP

    Lentiviral human MCFD2 shRNA (UAS,iRFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  12. HSH-115670-10ugiRFP

    Lentiviral human MCFD2 shRNA (UAS,iRFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 10 ug each
    $1,290.00

Product Details

Target gene information

Gene symbolMCFD2
SynonymsF5F8D F5F8D2 LMAN1IP SDNSF
Specieshuman
Gene ID90411
SummaryThis gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LMAN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2016].

shRNA lentiviral particle information

Functional titer1x10^8 TU/ml
Titration methodcounting puromycin resistant cell clones
PurityIn vivo grade
PseudotypeVSV-G
Storage bufferLentiShield™Lentivirus storage buffer
Storage condition-80°C
Availability3-4 weeks

shRNA lentiviral plasmid information

Target shRNAHuman MCFD2 shRNA
Promoter for target shRNAUAS
Mammalian selectionPuromycin
Promoter for mammalian selection genePGK
E.coli selectionAmp
Storage condition-20°C
Availability1-2 weeks

Lentiviral shRNA Gene Knockdown and Verification Kit

  1. Constitutive lentiviral shRNA
  2. Inducible lentiviral shRNA
  3. Lentiviral shRNA with UAS elements
  4. Tissue specific lentiviral shRNA
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