Lentiviral human CYP27B1 shRNA (UAS)

Catalog number
HSH-118705
Kit Components
Lentiviral particles or plasmids for 3 unique target specific shRNA and 1 scramble shRNA, RT-PCR primer mix for target specific gene and a housekeeping gene

To order

  1. HSH-118705-25

    Lentiviral human CYP27B1 shRNA (UAS) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  2. HSH-118705-100

    Lentiviral human CYP27B1 shRNA (UAS) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  3. HSH-118705-10ug

    Lentiviral human CYP27B1 shRNA (UAS) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 10 ug each
    $1,290.00
  4. HSH-118705-25GFP

    Lentiviral human CYP27B1 shRNA (UAS,GFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  5. HSH-118705-100GFP

    Lentiviral human CYP27B1 shRNA (UAS,GFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  6. HSH-118705-10ugGFP

    Lentiviral human CYP27B1 shRNA (UAS,GFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a GFP reporter, 10 ug each
    $1,290.00
  7. HSH-118705-25RFP

    Lentiviral human CYP27B1 shRNA (UAS,RFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  8. HSH-118705-100RFP

    Lentiviral human CYP27B1 shRNA (UAS,RFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  9. HSH-118705-10ugRFP

    Lentiviral human CYP27B1 shRNA (UAS,RFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a RFP reporter, 10 ug each
    $1,290.00
  10. HSH-118705-25iRFP

    Lentiviral human CYP27B1 shRNA (UAS,iRFP) (25)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 25 ul each, functional titer of 1x10^8 TU/ml
    $980.00
  11. HSH-118705-100iRFP

    Lentiviral human CYP27B1 shRNA (UAS,iRFP) (100)

    Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 4x25 ul each, functional titer of 1x10^8 TU/ml
    $2,190.00
  12. HSH-118705-10ugiRFP

    Lentiviral human CYP27B1 shRNA (UAS,iRFP) plasmid

    Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, with a miRFP670 reporter, 10 ug each
    $1,290.00

Product Details

Target gene information

Gene symbol
CYP27B1
Synonyms
CP2B CYP1 CYP1alpha CYP27B P450c1 PDDR VDD1 VDDR VDDRI VDR
Species
human
Gene ID
1594
Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008].

shRNA lentiviral particle information

Functional titer
1x10^8 TU/ml
Titration method
counting puromycin resistant cell clones
Purity
In vivo grade
Pseudotype
VSV-G
Storage buffer
LentiShield™Lentivirus storage buffer
Storage condition
-80°C
Availability
3-4 weeks

shRNA lentiviral plasmid information

Target shRNA
Human CYP27B1 shRNA
Promoter for target shRNA
UAS
Mammalian selection
Puromycin
Promoter for mammalian selection gene
PGK
E.coli selection
Amp
Storage condition
-20°C
Availability
1-2 weeks
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