Lentiviral particles or plasmids for 3 unique target specific shRNA and 1 scramble shRNA, RT-PCR primer mix for target specific gene and a housekeeping gene
Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 25 ul each, functional titer of 1x10^8 TU/ml
$890.00
HSH-118705-100
Lentiviral human CYP27B1 shRNA (UAS) (100)
Lentiviral particles for 3 unique target specific shRNA and 1 scramble shRNA driven by the UAS promoter, 4x25 ul each, functional titer of 1x10^8 TU/ml
$1,980.00
HSH-118705-10ug
Lentiviral human CYP27B1 shRNA (UAS) plasmid
Lentiviral constructs for 3 unique target specific shRNA and 1 scramble shRNA driven by the tet promoter, 10 ug each
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008].
shRNA lentiviral particle information
Functional titer
1x10^8 TU/ml
Titration method
counting puromycin resistant cell clones
Purity
N/A
Pseudotype
N/A
Storage buffer
LentiShield™Lentivirus storage buffer
Storage condition
-80°C
Availability
3-4 weeks
shRNA lentiviral plasmid information
Target shRNA
Human CYP27B1 shRNA
Promoter for target shRNA
UAS
Mammalian selection
Puromycin
Promoter for mammalian selection gene
PGK
E.coli selection
Amp
Storage condition
-20°C
Availability
1-2 weeks
Lentiviral shRNA Gene Knockdown and Verification Kit
