Overview
Lentiviral GFP-miR-shRNA cloning vector (tet, Puro) is a third generation lentiviral vector, with tetracycline-responsive promoter element (TRE) to drive GFP and miR-shRNA coexpression and PGK promoter to drive selection marker puromycin expression. This vector is compatible with both 2nd and 3rd generation packaging systems.
Clone your miR-shRNA into the Lentiviral GFP-miR-shRNA cloning vector (tet, Puro), then co-transfect with selected Lentivirus Packaging System into packaging cells, such as HEK293T cells to produce lentivirus. Lentivirus transduced cells can be selected by puromycin to generate stable cell lines.
Features:
- A third generation lentiviral vector
- Extremely Low recombination rate
- Compatible with 2nd and 3rd generation of packaging systems, ideal with our Lentivirus Packaging System.
Product Details
| Vector name | pLV-tet-GFP-miR-PGK-Puro |
| Package Size | 10 ug |
| E.coli selection | Amp |
| Promoter for target gene | TRE |
| Mammalian selection | Puromycin |
| Promoter for selection gene | PGK |
| Storage | -20°C |