Overview
Lentiviral Cloning Vector (EF1a,Hyg) is a third generation lentiviral vector, with EF1a promoter to drive your target gene expression and PGK promoter to drive selection marker hygromycin. This vector is compatible with both 2nd and 3rd generation of packaging systems.
Clone your gene of interest into the Multiple Cloning Sites (MCS), then co-transfect with selected Lentivirus Packaging System into packaging cells, such as HEK293T cells to produce lentivirus. Lentivirus transduced cells can be selected by hygromycin to generate stable cell lines.
Features:
- A third generation lentiviral vector
- High protein expression levels
- Extremely low recombination rate
- Compatible with 2nd and 3rd generation of packaging systems, ideal with our Lentivirus Packaging System
Product Details
| Vector name | pLV-EF1a-PGK-Hyg |
| Package Size | 10 ug |
| E.coli selection | Amp |
| Promoter for target gene | EF1a |
| Mammalian selection | Hygromycin |
| Promoter for selection gene | PGK |
| Storage | -20°C |