K-562/Luciferase cells are derived from the human K-562 lymphoblast cell line by stably integration of a constitutive Firefly luciferase stably expression construct. K-562 cell line was generated from chronic myelogenous leukemia, has been widely used in research to understand the molecular mechanisms underlying leukemia and to develop new treatment strategies. K-562/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.