Jurkat/GFP-luciferase cells are derived from the human immortalized T lymphocyte Jurkat cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. Jurkat cells have been used to study acute T cell leukemia, T cell signaling, and the stably expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.