HUTU 80/GFP-luciferase cells are derived from the human duodenum carcinoma HUTU 80 cell line by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. The HUTU 80 cell line has been widely used in cancer research and drug development. HUTU 80/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.