HL-60/Luciferase cells are derived from the human leukemia HL-60 cell line by stably integration of a constitutive Firefly luciferase stably expression construct. HL-60 cells have been used in cancer research and drug development. HL-60/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.