Hep3B/Luciferase cells are derived from the human Hep3B Hepatocellular carcinoma cell line by stably integration of a constitutive firefly luciferase stably expression construct. The Hep3B cell line has been widely used in cancer research, particularly in studies related to liver cancer, and drug screening. Hep3B/Luciferase cells stably stably express firefly luciferase, can be used for in vitro assays and in vivo imaging.