HEK293T/Luciferase cells are derived from human embryonic kidney cell line HEK293T by stably integration of a constitutive Firefly luciferase stably expression construct. HEK293T cells are widely used in biomedical research due to their robust growth, ease of transfection, and versatility in various experimental applications. HEK293T/Luciferase cells stably express Firefly luciferase, can be used for in vitro assays and in vivo imaging.