HEK293T/GFP cells are derived from human embryonic kidney cell line HEK293T by stably integration of a constitutive GFP stably expression construct. HEK293T cells are widely used in biomedical research due to their robust growth, ease of transfection, and versatility in various experimental applications. HEK293T/GFP cells stably express GFP, can be used for in vitro assays and in vivo imaging.