HEK293T/Cre cells are derived from human embryonic kidney cell line HEK293T by stably integration of a constitutive Cre recombinase stably expression construct. HEK293T cells are widely used in biomedical research due to their robust growth, ease of transfection, and versatility in various experimental applications. HEK293T/Cre cells stably express a nuclear localized Cre recombinase.