HEK 293 SMAD/TGFbeta Reporter (Luc) cells are derived from the human immortalized embryonic kidney cell line HEK 293 by stably integration of a SMAD/TGFbeta firefly luciferase reporter construct. HEK 293 cells have been widely used in cell biology research because of their reliable growth and propensity for transfection. HEK 293 SMAD/TGFbeta Reporter (Luc) cells stably express firefly luciferase under the control of the SMAD/TGFbeta response elements, can be used for monitoring the activity of TGF?/SMAD signaling pathway.