HEK 293/GFP-luciferase cells are derived from the human immortalized embryonic kidney cell line HEK 293 by stably integration of a constitutive GFP and Firefly luciferase stably expression construct. HEK 293 cells have been widely used in cell biology research because of their reliable growth and propensity for transfection. HEK 293/GFP-luciferase cells stably express GFP and Firefly luciferase, can be used for in vitro assays and in vivo imaging.