HCT-15/Luciferase cells are derived from the human colon cancer HCT-15 cell line by stably integration of a constitutive Firefly luciferase stably expression construct. The HCT-15 cell line has been extensively characterized and serves as a valuable tool for understanding the molecular mechanisms underlying colon cancer progression and for testing potential treatment. HCT-15/Luciferase cells stably stably express firefly luciferase, can be used for in vitro assays and in vivo imaging.