Fork-head box protein G1 (FOXG1) is an evolutionarily conserved, winged-helix transcriptional repressor that that regulates neurogenesis. The FOXG1 gene has been implicated in the congenital form of Rett syndrome (RTT), a severe neurodevelopmental disorder. FOXG1 lentivirus (TET-inducible, Puro) expresses human FOXG1 under the control of the Tet responsive promoter, with puromycin as a selection marker.